A novel strategy for global mapping of O-GlcNAc proteins and peptides using selective enzymatic deglycosylation, HILIC enrichment and mass spectrometry identification

Schematic overview of the new strategy for O-GlcNAc peptides enrichment and identification from human urine by the combination of selective enzymatic deglycosylation, HILIC enrichment using hydrophilic polymer modified-silica microparticles (pPEGMA-SMs) and mass spectrometry analysis.306