کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5141972 | 1495970 | 2017 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Partial structural studies of fucosylated chondroitin sulfate (FuCS) using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Partial structural studies of fucosylated chondroitin sulfate (FuCS) using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics Partial structural studies of fucosylated chondroitin sulfate (FuCS) using attenuated total reflection fourier transform infrared spectroscopy (ATR-FTIR) and chemometrics](/preview/png/5141972.png)
چکیده انگلیسی
The compound fucosylated chondroitin sulfate from three sea cucumber species Holothuria atra, Stichopus horrens, and Holothuria arenicola was extracted and purified using strong ion exchange chromatography. The structure and sulfation patterns on their fucose branches were partially characterized and compared using liquid, gas chromatography-mass spectrometry, infrared spectroscopy, and chemometrics. The monosaccharide composition was consistently a 1:1 molar ratio of glucuronic acid to N-acetylgalactosamine while the fucose molar ratio was statistically different among the three species. Normal IR spectra exhibited a broad band at the region of 850-830Â cmâ1 assigned to the sulfate COS group. This band was resolved in the second derivative spectra into two or three bands corresponding to the difference of sulfation patterns among sea cucumber species. Principal component analysis (PCA) confirmed the difference in the sulfate substitution region with additional discrimination of the combination amide NH and CO group, sulfate SO region, and skeletal region 1050-980Â cmâ1. The hierarchical clustering analysis (HCA) successfully grouped each sea cucumber FuCS in the same cluster. Nevertheless, the comparison of the FuCS structure was simplified using chemometrics analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 89, March 2017, Pages 26-36
Journal: Vibrational Spectroscopy - Volume 89, March 2017, Pages 26-36
نویسندگان
Pang Myron, Shafiquzzaman Siddiquee, Sujjat Al Azad,