کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5156966 | 1500593 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of O-chitosan nanoparticles on the development and membrane permeability of Verticillium dahliae
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کلمات کلیدی
Ethanol (PubChem CID: 702) - اتانول (PubChem CID: 702)Acetic acid (PubChem CID: 176) - اسید استیک (PubChem CID: 176)Oleic acid (PubChem CID: 445639) - اسید اولئیک (PubChem CID: 445639)Spore germination - جوانه زنی اسپورCell membrane - غشای سلولیFluorescein isothiocyanate (PubChem CID: 18730) - فلوورسین ایسوتیوسیانات (PubChem CID: 18730)Plant pathogenic fungi - قارچ بیماری های گیاهیMorphology - مورفولوژی(ریخت شناسی)Chitosan (PubChem CID: 71853) - کیتوزان (PubChem CID: 71853)Chitosan - کیتوسان Glutaraldehyde (Pubchem CID: 3485) - گلوتارالدئید (Pubchem CID: 3485)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Verticillium dahliae, which causes wilting in over 300 woody and herbaceous plant species, is a representative of fungal plant diseases for which effective controls are still needed. In this study, the antifungal action of oleoyl-chitosan nanoparticles was investigated against V. dahliae. Media containing oleoyl-chitosan nanoparticles dramatically decreased the mycelium growth. The highest antifungal indexes were observed on media amended with 2Â mg/mL nanoparticles. Optical microscopy showed that spore germination and hyphae morphology were affected. Scanning electron microscopy and transmission electron microscopy demonstrated degenerative alterations including crumpled hyphae and spores, thickened cell walls, disappeared membranous organelles, massive vacuolation of the cytoplasm, and cell wall-plasmalemma separation. Fluorescence microscopy showed that nanoparticles were internalized by fungal cells. The sharp increase in the release of intracellular components and decrease of total cellular protein concentration demonstrated damaged cell membranes. Overall, the results indicate that oleoyl-chitosan nanoparticles have the potential to control phytopathogens in agriculture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 165, 1 June 2017, Pages 334-343
Journal: Carbohydrate Polymers - Volume 165, 1 June 2017, Pages 334-343
نویسندگان
Ke Xing, Yuanfang Liu, Xiaoqiang Shen, Xiao Zhu, Xiaoyan Li, Xiangmin Miao, Zhaozhong Feng, Xue Peng, Sheng Qin,