کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5157746 1500597 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An injectable alginate-based hydrogel for microfluidic applications
ترجمه فارسی عنوان
یک هیدروژل مبتنی بر آلژینات تزریقی برای کاربردهای میکرو فلوئیدیک
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی
The objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of d-glucono-δ-lactone (GDL) and release of Ca+ ions from solid CaCO3. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100 μl spiral coil single channel microchip was fabricated and alginate GDL mixture containing β-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24 h without any leakage or deactivation of immobilized enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 161, 1 April 2017, Pages 228-234
نویسندگان
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