کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5157872 | 1500601 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extraction, characterization and biological activity of a (1,3)(1,6)-β-d-glucan from the pathogenic oomycete Pythium insidiosum
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کلمات کلیدی
Th17PythiosisDeuterium oxide (PubChem CID: 24602)β-D-glucan - β-D-گلوکانAcetone (PubChem CID: 180) - استون (PubChem CID: 180)Formic acid (PubChem CID: 284) - اسید فرمیک (PubChem CID: 284)Lymphocyte proliferation - تکثیر لنفوسیتDimethyl sulfoxide (PubChem CID: 679) - دی متیل سولفوکسید (PubChem CID: 679)Cytokines - سیتوکین هاFungal infection - عفونت قارچیChloroform (PubChem CID: 6212) - کلروفرم (PubChem CID: 6212)
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
Pythiosis is a life-threatening infectious disease caused by the pathogenic oomycete Pythium insidiosum. This study is the first to evaluate the P. insidiosum glucan content and its biological activities. The enzymatic quantification of the glucans in P. insidiosum mycelia showed that the β-glucan content was 18.99% ± 3.59. The cell wall polysaccharide extract consisted of â¼81.7% carbohydrates (exclusively glucose) and â¼18.3% residual amino acids and peptides. The results from monosaccharide composition, methylation and 1D/2D NMR spectroscopy analyses indicated the presence of a highly branched (1,3)(1,6)-β-d-glucan, with (1,6)-β-d-glucopyranosil side-branching unit on average every 1-2 repeat units. In vitro, the β-d-glucan extract could significantly promote spleen lymphocyte proliferation in human, equine and mouse cell cultures. BALB/c mice that were subcutaneously pre-immunized with three doses of 0.5, 2.5 and 5.0 mg of β-glucan/mouse, showed a significant increase in IL-2, IL-6, IL-10, TNF-α and IL-17A production compared to non-immunized mice. These results suggested that β-d-glucan extract induces significant and specific Th17 cellular immune response and provided the theoretical basis for further experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 157, 10 February 2017, Pages 719-727
Journal: Carbohydrate Polymers - Volume 157, 10 February 2017, Pages 719-727
نویسندگان
Juliana S.M. Tondolo, Pauline C. Ledur, Ãrico S. Loreto, Camila M. Verdi, Paula E.R. Bitencourt, Francielli P.K. de Jesus, Jean P. Rocha, Sydney H. Alves, Guilherme L. Sassaki, Janio M. Santurio,