کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5160447 | 1501681 | 2017 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Milk β-casein as a vehicle for delivery of bis(indolyl)methane: Spectroscopy and molecular docking studies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Milk β-casein as a vehicle for delivery of bis(indolyl)methane: Spectroscopy and molecular docking studies Milk β-casein as a vehicle for delivery of bis(indolyl)methane: Spectroscopy and molecular docking studies](/preview/png/5160447.png)
چکیده انگلیسی
The interaction of bis(indolyl)methane with bovine milk β-casein was investigated using spectroscopy and molecular docking studies at different temperatures (25-37 °C). The circular dichroism and Fourier transform infrared spectroscopic data demonstrated that β-casein interacts with BIM molecule mainly via both the hydrophobic and hydrophilic interactions with a minor change in the secondary structure of β-casein. The fluorescence quenching measurements revealed that the presence of a single binding site on β-casein for BIM with the binding constant value of â¼104 Mâ1. The negative values of entropy and enthalpy changes confirm the predominate role of hydrogen binding and van der Waals interactions in the binding process. FÓ§rster energy transfer measurement suggested that the distance between bound BIM and Trp residue is higher than the respective critical distance. Hence, the static quenching is more likely responsible for the fluorescence quenching rather than the mechanism of non-radiative. Docking study showed that BIM molecule forms three hydrogen bonds and several van der Waals contacts with β-casein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 1136, 15 May 2017, Pages 50-58
Journal: Journal of Molecular Structure - Volume 1136, 15 May 2017, Pages 50-58
نویسندگان
Hamid Dezhampanah, Masoomeh Esmaili, Alireza Khorshidi,