Molecular cloning and characterization of a Perilla frutescens cytochrome P450 enzyme that catalyzes the later steps of perillaldehyde biosynthesis

A cytochrome P450 type enzyme with perillyl alcohol and perillaldehyde synthases activities was isolated by analyzing an expressed sequence tag library from several oil types of pure lines of perilla. The recombinant protein catalyzed the hydroxylation and oxidation of limonene to perillyl alcohol and perillaldehyde.116