Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells

► A cDNA of P450 in sanguinarine biosynthesis was isolated from Eschscholzia californica. ► Recombinant P450 (CYP82N2v2) was produced in Saccharomyces cerevisiae. ► Recombinant CYP82N2v2 showed protopine conversion to produce dihydrosanguinarine. ► Recombinant CYP82N2v2 also showed allocryptopine conversion to produce dihydrochelerythrine. ► Characterization of the substrate-specificity supported the 6-hydroxylation in the reaction.