Sequence analysis and structure prediction of enoyl-CoA hydratase from Avicennia marina: Implication of various amino acid residues on substrate-enzyme interactions

Homology model of Avicennia marina enoyl-CoA hydratase mainly comprises α-helical regions. Glu106 is the conserved catalytic residue while Glu86 is replaced by Gly. Asp114 is proposed as the second catalytic residue. There were structural variations in mobile loop and formation and loss of interactions between active site and substrate.