Histolocalization and physico-chemical characterization of dihydrochalcones: Insight into the role of apple major flavonoids

► Easy staining methods for dihydrochalcone location in apple tissues are proposed. ► Dihydrochalcones localize in upper layers of leaves and around vascular bundles. ► Phloretin shows a high antimicrobial activity against a broad spectrum of microbes. ► The o-diphenol sieboldin is a potent iron-chelator.