High-mannose N-glycan-specific lectin from the red alga Kappaphycus striatum (Carrageenophyte)

► Three isolectins (KSA-1-3) were isolated from the cultivated Kappaphycus striatum. ► KSAs are monomeric proteins of about 28 kDa having identical N-terminal sequences. ► KSAs have affinity for high-mannose-linked glycoproteins but not for monosaccharides. ► KSA-2 shows the strict binding-specificity for high mannose N-glycans. ► KSAs are evaluated with a unique carbohydrate probe, promising their medicinal uses.