Proteomic approach to characterize mitochondrial complex I from plants

► Mitochondrial complex I is the largest protein complex of the respiratory chain. ► Proteomic analyses recently allowed to explore mitochondrial complex I from plants. ► The plant enzyme is especially large and includes 15 extra subunits. ► Some of them are enzymes (e.g. carbonic anhydrases) introducing side activities to plant complex I. ► This article reviews and re-evaluates proteomic data on the protein composition of plant complex I.