Substrate specificities of family 1 UGTs gained by domain swapping

Chimeric family 1 UDP-glucose glycosyltransferases (UGTs) with substrates specificities were constructed. Twelve active chimeras were obtained, using domains of the UGTs 71C1, 71C2, 71E1 and 85C1, of two different plant species. Among activities observed were regio-specific glycosylation of resveratrol and increased efficiency of etoposide glycosylation.