Class targeted metabolomics: ESI ion trap screening methods for glucosinolates based on MSn fragmentation

Glucosinolates are naturally occurring anionic secondary plant metabolites that generate a sulphated-glycoside during mass spectrometry fragmentation. This fragment ion can be utilised in qualitative and quantitative LCMSn analysis and also in a rapid, highly sensitive parent ion mapping experiment to allow class targeted analysis of glucosinolates in crude plant extracts.