Determination of saponins in Maesa lanceolata by LC-UV: Development and validation

A method is described to quantify the saponins in Maesa lanceolata extracts. The method is based on a H2O/MeOH extraction and a purification step on a C18 SPE cartridge. The identification is done by the combination of LC-UV/MS, while quantification is performed by LC-UV, using oleanolic acid as an external standard.