Calliandra selloi Macbride trypsin inhibitor: Isolation, characterization, stability, spectroscopic analyses

A Kunitz-type trypsin inhibitor was purified from Calliandra selloi seeds. Circular dichroism analyses, fluorescence and thermal denaturation studies clearly demonstrated that the protein was rich in beta-sheet structure, highly stable over a wide range of pH and temperature, but labile to reduction and carbamidomethylation.