Poly (butylene adipate-co-terephthalate)/hydroxyapatite composite structures for bone tissue recovery

Tissue engineering is a multidisciplinary science that offers a strategy to circumvent the problems related with regenerative and therapeutic procedures. The development of biomaterials made of biodegradable polymers and hydroxyapatite (HA) has been extensively investigated to create biological substitutes to regenerate and repair bone tissue. In this research, a bionanocomposite scaffold based on poly (butylene adipate-co-terephthalate) (PBAT) and HA nanoparticles was prepared by electrospinning and spin coating techniques. The characterization of the composite structures was made by scanning and transmission electron microscopy (SEM and TEM), differential scanning calorimetry (DSC), Fourier Transform infrared (FTIR), wide angle X-rays diffraction (WAXD) and tensile mechanical properties, which were measured by dynamical mechanical analysis (DMA). Afterwards, human adipose stem cells (hASC) were seeded over the composite material and its differentiation in osteoblasts and in vivo biocompatibility were evaluated. This study showed that the composite material had a proper morphology, structure and mechanical properties which ensured the hASC attachment, proliferation and differentiation in bone cells. Finally, as implants, the composite material triggered only a mild inflammatory response.