کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5209896 | 1503074 | 2015 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase](/preview/png/5209896.png)
چکیده انگلیسی
This work aims to reuse pyranose 2-oxidase (P2O) and improve its thermal stability compared to the wild-type enzyme by immobilizing onto regenerated cellulose fibers (RC). The P2O-immobilized RC fibers (P2O-RC) showed the improvement in terms of thermal stability. Cellulose acetate (CA) fibers (229 ± 42 nm) were electrospun from 17% w/w CA (MW â¼3 Ã 104 g/mol) with 5% w/w Tween 80 dissolved in a mixture of water (25% w/w) and acetic acid (75% w/w) at an applied voltage of 25 kV and a fiber collection distance of 10 cm. Then, the fiber surface was treated with 0.5 M potassium hydroxide in ethanol and 12% w/v glutaraldehyde using aluminum sulfate hexadecahydrate as a catalyst before P2O was immobilized on the treated fibers. Effects of the immobilization process on thermal and pH stability of P2O were investigated by measuring P2O activity based on peroxidase coupled assay. Results indicated that P2O-RC catalyzed the oxidation of glucose at high temperatures (60 and 70 °C) better than the free enzyme did. Therefore, P2O-RC are potentially useful as a heterogeneous catalyst under the conditions that P2O cannot endure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 86, January 2015, Pages 47-51
Journal: Reactive and Functional Polymers - Volume 86, January 2015, Pages 47-51
نویسندگان
Weerapha Panatdasirisuk, Thammasit Vongsetskul, Jeerus Sucharitakul, Pimchai Chaiyen, Pramuan Tangboriboonrat,