کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5211038 | 1382933 | 2008 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thermoresponsive-fluorescent isopropylacrylamide-vinylpyridine-pyrenemethylacrylamide terpolymer and its complexation behaviour with DNA
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کلمات کلیدی
DNA - DNA یا اسید دزوکسی ریبونوکلئیکGene delivery - انتقال ژن Isopropylacrylamide - ایزوپروپیل آکریل آمیدFluorescence spectroscopy - فوتولومینسانس یا فلوئورسانس یا فسفرسانسVinylpyridine - وینیل پرییدینThermosensitive polymer - پلیمر حرارتی حساسStimuli-sensitive polymers - پلیمرهای حساس محرکPyrene - پیرنه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Temperature and pH-responsive, fluorescent random terpolymer of N-isopropylacrylamide (NIPA), 4-vinylpyridine (4VP) and N-pyrenemethylacrylamide (PyMeA) was synthesized. Temperature and pH-dependent fluorescence behaviour of poly(NIPA-4VP-PyMeA) terpolymer was investigated by fluorescence spectroscopy. The protonation of terpolymer (i.e. high cationic charge) in the acidic pH region provided significantly higher values for the ratio of the first to third vibronic band intensities (I1/I3) with respect to that found for pyrene in an aqueous medium (i.e. 1.87). I1/I3 ratio significantly decreased with increasing pH depending upon the decreasing polarity of microenvironment due to the deprotonation of terpolymer. As a new potential carrier for DNA-delivery, the interaction of fluorescent poly(NIPA-4VP-PyMeA) terpolymer with DNA was investigated. The addition of DNA into the fluorescent terpolymer solution resulted in the formation of stable, colloidal terpolymer-DNA complex due to the bridge formation between phosphate groups of DNA and protonated 4VP units of the fluorescent terpolymer. The hydrodynamic diameter of DNA-poly(NIPA-4VP-PyMeA) terpolymer complex increased with increasing DNA concentration. The formation of colloidal complex particles lower than 1 μm at the pHs close to the physiological pH value showed that poly(NIPA-VP-PyMeA) terpolymer could be utilized as a new fluorescent non-viral vector for DNA delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 68, Issue 2, February 2008, Pages 623-633
Journal: Reactive and Functional Polymers - Volume 68, Issue 2, February 2008, Pages 623-633
نویسندگان
Kadir Utku Ãzen, Begum Elmas, Ãzgür Ãzsar, Serap Senel, Ali Tuncel,