کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5211612 | 1382960 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Semi-interpenetrating polymer networks (IPNs) for entrapment of glucose isomerase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Semi-interpenetrating polymer networks (IPNs) for entrapment of glucose isomerase Semi-interpenetrating polymer networks (IPNs) for entrapment of glucose isomerase](/preview/png/5211612.png)
چکیده انگلیسی
Glucose isomerase (GI) was entrapped in three different hydrogels such as poly(acrylamide), semi-interpenetrating poly(acrylamide)/κ-carrageenan, and poly(acrylamide)/alginate polymer networks. The values for pH optimum and temperature for free and immobilized glucose isomerase were found to be the same as 7.5 and 60 °C, respectively. The Km values for free and immobilized enzyme in poly(acrylamide), poly(acrylamide)/κ-carrageenan and poly(acrylamide)/alginate matrices were determined as 18.87, 1.22, 2.78, and 4.54 mg/mL, respectively, while the Vmax values for the same systems calculated as 2.51, 0.63, 0.72, and 0.82 mg/mL min, respectively. The storage stability values of immobilized enzyme systems were observed as 81%, 33% and 32%, respectively, after 42 days. In addition to this, it was observed that, after 25th use in 5 days, the retained activities for immobilized enzyme in poly(acrylamide), and semi-interpenetrating polymer networks of poly(acrylamide)/κ-carrageenan and poly(acrylamide)/alginate matrices were found as 98%, 71% and 72%, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reactive and Functional Polymers - Volume 66, Issue 4, April 2006, Pages 389-394
Journal: Reactive and Functional Polymers - Volume 66, Issue 4, April 2006, Pages 389-394
نویسندگان
G. Demirel, G. Ãzçetin, F. Åahin, H. Tümtürk, S. Aksoy, N. Hasirci,