کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5371640 1503958 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding affinity and mode of distamycin A with A/T stretches in double-stranded DNA: Importance of the terminal A/T residues
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Binding affinity and mode of distamycin A with A/T stretches in double-stranded DNA: Importance of the terminal A/T residues
چکیده انگلیسی

Distamycin A (Dst) is an antibiotic which binds to the minor groove of double-stranded DNA at A/T-rich regions. We have examined the affinity and mode of Dst binding to DNA duplexes containing a conserved A/T core and variable terminal A/T regions by using circular dichroism spectroscopy. The observed circular dichroism spectra were analyzed by singular value decomposition and fitted to a two-step binding model. The result clearly shows a correlation between the affinity for Dst and the preference for Dst-DNA 1:1 binding over 2:1 binding. The A/T stretches that prefer 1:1 binding form high-affinity 1:1 complexes, whereas those preferring 2:1 binding form stable 2:1 complex with low overall affinities. The terminal A/T residues of the Dst binding region play an important role in the stabilization/destabilization of the 1:1 and 2:1 complexes, resulting in a terminal residue-dependent variation of the binding affinity and the binding mode preference.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biophysical Chemistry - Volume 149, Issues 1–2, June 2010, Pages 34-39
نویسندگان
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