کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5395866 | 1505737 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A NEXAFS and mass spectrometry study of cysteine, cystine and insulin irradiated with intermediate energy (0.8Â keV) electrons
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A NEXAFS and mass spectrometry study of cysteine, cystine and insulin irradiated with intermediate energy (0.8Â keV) electrons A NEXAFS and mass spectrometry study of cysteine, cystine and insulin irradiated with intermediate energy (0.8Â keV) electrons](/preview/png/5395866.png)
چکیده انگلیسی
We have performed a NEXAFS (S 1s) and mass spectrometry study of solid samples of cysteine, cystine and insulin irradiated with 0.8Â keV electrons. The measured mass spectra point out to processes of desulfurization, deamination, decarbonylation and decarboxylation in the irradiated biomolecules. Electron beam irradiation was also conducted at low temperatures in order to evaluate the possible contribution from thermal degradation processes. The NEXAFS spectra of irradiated cysteine and cystine did not show substantial changes when compared to the same spectra obtained using non-irradiated samples. The sulfur K-edge photoabsorption spectrum of irradiated insulin, however, showed clear modifications when compared to the spectrum of the non-irradiated protein, even when the irradiation was conducted at low temperature. Using an empirical combination of the photoabsorption spectra of cysteine and cystine (which are associated respectively with reduced and oxidized forms of sulfur) we have been able to reproduce the absorption spectrum of irradiated insulin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electron Spectroscopy and Related Phenomena - Volume 193, March 2014, Pages 21-26
Journal: Journal of Electron Spectroscopy and Related Phenomena - Volume 193, March 2014, Pages 21-26
نویسندگان
G. Simões, F.N. Rodrigues, R.B. Bernini, C.S.C. Castro, G.G.B. de Souza,