کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5400085 | 1505918 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of an anticancer Rutaceae plant flavonoid glycoside with calf thymus DNA: Biophysical and electrochemical studies
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
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چکیده انگلیسی
In the present work, we report the interaction of a bioactive Rutaceae plant flavonoid glycoside, diosmin (DIO) with calf thymus DNA employing ethidium bromide as a fluorescence probe. The mode of binding between DIO and DNA was investigated by UV absorption, fluorescence, 3D-fluorescence, fluorescence polarization, FT-IR, circular dichroism, melting temperature (Tm) measurements and differential pulse voltammogram studies. The results revealed the intercalative mode of binding between DIO and DNA. Further, the values of thermodynamic parameters, âH° (â388.32 kJ molâ1) and âS° (â1.22 kJ molâ1 Kâ1) indicated that the van der Waals forces and hydrogen bond played a major role in the binding of DIO to DNA. The observed negative âG° values revealed the spontaneity of interaction process. The binding of DIO to DNA-EB was found to be stronger in the presence of coexisting substances.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 142, October 2013, Pages 17-22
Journal: Journal of Luminescence - Volume 142, October 2013, Pages 17-22
نویسندگان
Sandhya Balakrishnan, Seetharamappa Jaldappagari,