کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5400401 | 1505920 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Studies on the interactions of chloroquine diphosphate and phenelzine sulfate drugs with human serum albumin and human hemoglobin proteins by spectroscopic techniques
ترجمه فارسی عنوان
مطالعات بر روی تعاملات داروهای کلروکین دی فسفات و فنلزین سولفات با آلبومین سرم انسان و پروتئین های هموگلوبین انسان با روش های اسپکتروسکوپی
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کلمات کلیدی
آلبومین سرم انسان، هموگلوبین، اثر متقابل، کلروفین دی فسفات، فنلزین سولفات، طیف سنجی فلورسانس،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
چکیده انگلیسی
The interactions of chloroquine diphosphate (CQP) and phenelzine sulfate (PS) drugs with human serum albumin (HSA) and human hemoglobin (HMG) proteins were investigated by various spectroscopic methods. It was found that CQP caused the fluorescence quenching of protein molecules through a static quenching mechanism, but PS did not. The values of Stern-Volmer quenching constant, bimolecular quenching constant, binding constant and number of binding site on the protein molecules were calculated for HSA-CQP and HMG-CQP systems at pH 7.4 and different temperatures. For CQP, there was only one binding site on HSA and HMG proteins and the binding affinity of HSA was higher than that of HMG. The binding constants decreased with increasing temperature. The values of negative enthalpy change and positive entropy change indicated that electrostatic interactions play an important role in the binding processes. In addition, the binding processes were spontaneous and carried out by exothermic reactions. According to Förster resonance energy transfer theory, the average binding distance between proteins and CQP was calculated as 3.72 nm for HSA-CQP system and 3.45 nm for HMG-CQP system. Circular dichroism analysis displayed that the addition of CQP led to a decrease in the α-helix amount of HSA and HMG proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 140, August 2013, Pages 87-94
Journal: Journal of Luminescence - Volume 140, August 2013, Pages 87-94
نویسندگان
Sibel Tunç, Osman Duman, Bahar Kancı BozoÄlan,