کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5400425 | 1505917 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Photoluminescence studies of a Terbium(III) complex as a fluorescent probe for DNA detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The photoluminescence properties of a Tb(III) complex of the form [Tb(phen)2Cl3·OH2] (phen=1,10-phenanthroline) in different solvents are presented. It shows the characteristic luminescence of the corresponding Ln3+ ion in the visible region. The emission intensity of this complex in coordinating solvent is higher than non-coordinating one. The suggested mechanism for the energy transfer between the ligand and Tb3+ ion is the intramolecular energy transfer mechanism. The interactions of the Tb(III) complex with fish salmon DNA are studied by fluorescence spectroscopy, circular dichroism study and viscosity measurements. The results of fluorescence titration reveal that DNA strongly quenches the intrinsic fluorescence of the complex through a static quenching procedure. The binding constant (Kb) of the above metal complex at 25 °C is determined by the fluorescence titration method and it is found to be (8.06±0.01)Ã103 Mâ1. The thermodynamic parameters (ÎH0>0, ÎS0>0 and ÎG0<0) indicate that the hydrophobic interactions play a major role in DNA-Tb complex association. The results support the claim that the title complex bonds to FS-DNA by a groove mode.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Luminescence - Volume 143, November 2013, Pages 56-62
Journal: Journal of Luminescence - Volume 143, November 2013, Pages 56-62
نویسندگان
Mozhgan Khorasani-Motlagh, Meissam Noroozifar, Sona Niroomand, Asieh Moodi,