Studies on the interaction between scopoletin and two serum albumins by spectroscopic methods

► Binding modes of scopoletin to HSA/BSA have been established. ► The binding sites on BSA/HSA by scopoletin were discussed. ► Investigating the structural changes of HSA and BSA in the presence of scopoletin. ► Energy transfer and the type of the binding forces were investigated for two systems. ► Influences of common ions on the binding constants of BSA/HSA with scopoletin were investigated.