Specificity and affinity of phenosafranine protein adduct: Insights from biophysical aspects

► Our study supply salient clues on the toxicological action of toxic phenosafranine. ► Phenosafranine is situated within subdomain IIIA, Sudlow's site II on HSA. ► Trp-62, Trp-63 and Trp-108 residues on the Lys molecule are all close to dye. ► Static property of the phenosafranine induced quenching of protein Trp fluorescence. ► Protein spatial structure proved to be disturbed after complex with phenosafranine.