Spectroscopic investigation on the sonodynamic activity of amsacrine (AMSA) to bovine serum albumin (BSA) damage

► AMSA is used as sonosensitizer to study the sonodynamic damage to BSA. ► Synchronous fluorescence spectroscopy is used to study AMSA binding site to BSA. ► Synchronous fluorescence spectroscopy is used to study AMSA damage site to BSA. ► ROS was estimated by the method of oxidation-extraction spectrometry (OES).