ReviewStudies on the arctiin and its interaction with DNA by spectral methods

The emission spectra of arctiin were determined under various experimental conditions. In addition, a fluorescence method was developed to obtain the binding constants and sites of the interaction between arctiin and DNA. A competitive binding experiment and melting temperature mensuration were carried out to investigate the binding mechanism of arctiin and DNA. The experimental results showed that the interaction between arctiin and DNA belongs to a groove binding mode.

► Determined the emission spectra of arctiin by fluorescence spectrometry. ► Obtain the binding constants and sites of interaction between arctiin and DNA. ► Calculate the binding parameters according an improved calculation method.