Application of Box-Behnken design in the optimization of catalytic behavior of a new mixed chelate of copper (ІІ) complex in chemiluminescence reaction of luminol

► In this study we introduce a new mixed chelate of copper complex as a catalyst of luminol chemiluminescence (CL) reaction. ► The copper complex (N-(2-(2-aminoethylamino)ethyl)-1H-pyrrole-2-carboxamide-Cu(II)) catalyst luminol reaction more than that of copper acetate and potassium hexacyanoferrate (III). ► The Box-Behnken design matrix and response surface methodology are used for prediction of CL intensity of luminol. ► There are good correlation between experimental and expected CL intensity that predicted by the theoretical model. ► Fluorescein used as a fluorescer in the luminol CL reaction in presence of the new catalyst.