Current applications of 19F NMR to studies of protein structure and dynamics

► 19F molecular tags and labeling protocols for 19F NMR studies of proteins are reviewed and contrasted. ► 19F NMR biosynthetic labeling strategies are presented. ► Experimental challenges (loss of function through labeling, line broadening, assignment ambiguities) are discussed. ► Approaches to the study of protein topology, using 19F NMR, are presented. ► Current examples of protein NMR studies are given.