Development of immunosensor using magnetic nanoparticles and circular microchannels in PDMS

• Effectively concentrating bacteria by MNP based immunoassay using microfluidics.
• Microchannels are fabricated easily and reproducibly in one step.
• Different concentrations of Escherichia coli were detected with high degree of repeatability.
• Inexpensive yet powerful tool to quantify bacteria is demonstrated.

Here we report development of a magnetic nanoparticles based immunosensor chip comprising of specially designed circular microchannel and embedded miniature permanent magnet. The present process of fabrication allows a rapid and reliable tool to create microchannels with ease and at a low cost. The chip permits easy in-flow capture of bacteria, their visual detection and quantification as demonstrated by Escherichia coli detection. Such a technique enhances capture efficiency and makes quantification of captured antigens simple.

One step, easy process was employed to fabricate circular micro-capillary with permanent magnet embedded in PDMS. The chip was tested successfully as biosensor for the detection of bacteria using magnetic nanoparticles.Figure optionsDownload as PowerPoint slide