کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5499324 | 1533496 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Non-DSB clustered DNA lesions. Does theory colocalize with the experiment?
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کلمات کلیدی
LMDSMonte Carlo N-particleNIRRBEPCCMCNPDSBRDDRNHEJMOCBERDSBsNER - DOWNROS - ROSSSBs - SSBRelative biological effectiveness - اثربخشی بیولوژیکی نسبیLET - اجازه دهیدNear infrared radiation - اشعه مادون قرمز نزدیکیLinear Energy Transfer - انتقال انرژی خطیionizing radiation - تابش یوننده یا پرتوهای یونیزانDNA repair - ترمیم DNADouble-strand break repair - تعمیر دوبار رشتهnucleotide excision repair - تعمیر مجدد نوکلئوتیدیbase excision repair - تعمیر پایه پایهDNA double strand breaks - دو رشته DNA شکسته می شودMonte Carlo - روش مونت کارلوsingle strand breaks - شکاف تک رشته ایPearson’s correlation coefficient - ضریب همبستگی پیرسونnon-homologous end joining - عدم پیوستن انتهای غیر همولوگFluorescence microscopy - میکروسکوپ فلورسانسHomologous recombination - نوترکیبی همولوگDNA damage response - واکنش به آسیب DNA Radiation therapy - پرتودرمانی یا رادیوتراپیRif - کد عکسReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
تشعشع
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ionizing radiation results in various kinds of DNA lesions such as double strand breaks (DSBs) and other non-DSB base lesions. These lesions may be formed in close proximity (i.e., within a few nanometers) resulting in clustered types of DNA lesions. These damage clusters are considered the fingerprint of ionizing radiation, notably charged particles of high linear energy transfer (LET). Accumulating theoretical and experimental evidence suggests that the induction of these clustered lesions appears under various irradiation conditions but also as a result of high levels of oxidative stress. The biological significance of these clustered DNA lesions pertains to the inability of cells to process them efficiently compared to isolated DNA lesions. The results in the case of unsuccessful or erroneous repair can vary from mutations up to chromosomal instability. In this mini review, we discuss of several Monte Carlo simulations codes and experimental evidence regarding the induction and repair of radiation-induced non-DSB complex DNA lesions. We also critically present the most widely used methodologies (i.e., gel electrophoresis and fluorescence microscopy [in situ colocalization assays]). Based on the comparison of different approaches, we provide examples and suggestions for the improved detection of these lesions in situ. Based on the current status of knowledge, we conclude that there is a great need for improvement of the detection techniques at the cellular or tissue level, which will provide valuable information for understanding the mechanisms used by the cell to process clustered DNA lesions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Radiation Physics and Chemistry - Volume 128, November 2016, Pages 26-35
Journal: Radiation Physics and Chemistry - Volume 128, November 2016, Pages 26-35
نویسندگان
Zacharenia Nikitaki, Vladimir Nikolov, Ifigeneia V. Mavragani, Ianik Plante, Dimitris Emfietzoglou, George Iliakis, Alexandros G. Georgakilas,