کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505281 | 1400264 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunophotoaffinity labeling of binders of 1-methyladenine, the oocyte maturation-inducing hormone of starfish
ترجمه فارسی عنوان
برچسب زدن ایمونوفوتوپتیک کننده گیرنده های 1-متیل آدنین، هورمون القاء بلوغ یخ زده ستاره دریایی
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کلمات کلیدی
HEPESGVBDMIHASWPMSFPAGECBBMPFSDSEGTATLCKLHEC501-methyladenine - 1-متیل آدنین2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid - 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acidcoomassie brilliant blue R-250 - coomassie درخشان آبی R-250polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدoocyte maturation - بلوغ تخمک یا بلوغ اووسیتmaturation-promoting factor - بلوغ فاکتور ترویجStarfish - ستاره دریاییsodium dodecyl sulfate - سدیم دودسیل سولفاتPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMaturation-inducing hormone - هورمون القاء بلوغthin-layer chromatography - کروماتوگرافی نازک لایهkeyhole limpet hemocyanin - کلم هول limpet هموسیانینReceptor - گیرنده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary and resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative 1-MeAde receptors on the oocyte surface have been suggested, but not yet been biochemically characterized. Immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection, was attempted to detect unknown 1-MeAde binders including putative maturation-inducing hormone receptors in starfish oocytes. When the oocyte crude membrane fraction or its Triton X-100/EDTA extract was incubated with N6-[6-(5-azido-2-nitrobenzoyl)aminohexyl]carboxamidomethyl-1-methyladenine and then photo-irradiated, followed by western blotting with antibody that was raised against a 1-MeAde hapten, a single band with Mr of 47.5 K was detected. The band was lost when extract was heated at 100 °C. A similar 47.5 K band was detected in the crude membrane fraction of testis as well. Upon labeling with whole cells, this band was detected in immature and maturing oocytes, but only faintly in mature oocytes. As judged from these results, this 1-MeAde binder might be a possible candidate of the starfish maturation-inducing hormone receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 485, Issue 1, 25 March 2017, Pages 41-46
Journal: Biochemical and Biophysical Research Communications - Volume 485, Issue 1, 25 March 2017, Pages 41-46
نویسندگان
Tetsuo Toraya, Tetsuo Kida, Atsushi Kuyama, Shinjiro Matsuda, Seiichi Tanaka, Yo Komatsu, Taro Tsurukai,