کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5505290 1400264 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of serotonin concentrations in human milk by high-performance liquid chromatography with fluorescence detection
ترجمه فارسی عنوان
تجزیه و تحلیل غلظت سروتونین در شیر انسان با استفاده از کروماتوگرافی مایع با عملکرد بالا با تشخیص فلورسانس
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Serotonin (5-hydroxytryptamine, 5-HT) plays an important role in milk volume homeostasis in the mammary gland during lactation; 5-HT in milk may also affect infant development. However, there are few reports on 5-HT concentrations in human breast milk. To address this issue, we developed a simple method based on high-performance liquid chromatography with fluorescence detection (HPLC-FD) for measuring 5-HT concentrations in human breast milk. Breast milk samples were provided by four healthy Japanese women. Calibration curves for 5-HT in each sample were prepared with the standard addition method between 5 and 1000 ng/ml, and all had correlation coefficients >0.999. The recovery of 5-HT was 96.1%-101.0%, with a coefficient of variation of 3.39%-8.62%. The range of 5-HT concentrations estimated from the calibration curves was 11.1-51.1 ng/ml. Thus, the HPLC-FD method described here can effectively extract 5-HT from human breast milk with high reproducibility.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 485, Issue 1, 25 March 2017, Pages 102-106
نویسندگان
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