کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505332 | 1400265 | 2017 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resveratrol induces membrane and DNA disruption via pro-oxidant activity against Salmonella typhimurium
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کلمات کلیدی
DAPIDTNBMDANPNGSSGGSHNACMICN-phenyl-1-naphthylamine2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetate4′,6-diamidino-2-phenylindole dihydrochloride - 4 '، 6-دی آمیدین-2-فنیلینول دی هیدروکلراید5,5′-dithiobis(2-nitrobenzoic acid) - 5،5'-dithiobis (2-nitrobenzoic acid)DiBAC4(3) - DiBAC4 (3)H2DCFDA - H2DCFD بهN-acetylcysteine - N-استیل سیستئینROS - ROSTerminal deoxynucleotidyl transferase dUTP nick end labeling - ترمینال deoxynucleotidyl transferase dUTP نام نهایی پایان نامهOxidative stress - تنش اکسیداتیوTUNEL - تونلMinimum inhibitory concentration - حداقل غلظت مهاریResveratrol - رسوراترولSalmonella Typhimurium - سالمونلا تیفی موریومOuter membrane - غشای خارجیAntibacterial activity - فعالیت ضد باکتریmalondialdehyde - مالون دی آلدهیدProoxidant - پروکسیدانreduced glutathione - کاهش گلوتاتیونoxidized glutathione - گلوتاتیون اکسید شدهReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Resveratrol is a flavonoid found in various plants including grapes, which has been reported to be active against various pathogenic bacteria. However, antibacterial effects and mechanisms via pro-oxidant property of resveratrol remain unknown and speculative. This research investigated antibacterial mechanism of resveratrol against a food-borne human pathogen Salmonella typhimurium, and confirmed the cell death associated oxidative damage. Resveratrol increased outer membrane permeability and membrane depolarization. It also was observed DNA injury responses such as DNA fragmentation, increasing DNA contents and cell division inhibition. Intracellular ROS accumulation, GSH depletion and significant increased malondialdehyde levels were confirmed, which indicated pro-oxidant activity of resveratrol and oxidative stress. Furthermore, the observed lethal damages were reduced by antioxidant N-acetylcysteine treatment supported the view that resveratrol-induced oxidative stress stimulated S. typhimurium cell death. In conclusion, this study expands understanding on role of pro-oxidant property and insight into previously unrecognized oxygen-dependent anti-Salmonella mechanism on resveratrol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 489, Issue 2, 22 July 2017, Pages 228-234
Journal: Biochemical and Biophysical Research Communications - Volume 489, Issue 2, 22 July 2017, Pages 228-234
نویسندگان
Wonjong Lee, Dong Gun Lee,