کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505373 | 1400267 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypotonicity activates a voltage-dependent membrane conductance in N2a neuroblastoma cells
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کلمات کلیدی
NMDGN-methyl-d-glucamineNppb5-nitro-2-(3-phenylpropylamino)benzoic acid - 5-نیترو-2- (3-phenylpropylamino) بنزوئیک اسیدHypotonic stress - استرس هیپوتیونیCell swelling - تورم سلولیTail current - جرقه جرقهVRAC - فلهVoltage-gated - ولتاژ خاموشvolume-regulated anion channel - کانال آنیونی تنظیم حجمIon channel - کانال یونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hypotonicity activates a voltage-dependent membrane conductance in N2a neuroblastoma cells Hypotonicity activates a voltage-dependent membrane conductance in N2a neuroblastoma cells](/preview/png/5505373.png)
چکیده انگلیسی
To maintain cellular and bodily homeostasis, cells respond to extracellular stimuli including osmotic stress by activating various ion channels, which have been implicated in many physiological and pathophysiological conditions. However, cellular osmosensory mechanisms remain elusive. Here, we report a novel voltage-dependent current in N2a cells activated by exposure to hypotonic stress. After a hypotonic challenge, N2a cells sequentially develop two distinct currents. The volume-regulated anion channel (VRAC) current emerges first and, after a delay, activation of a previously uncharacterized strongly outwardly rectifying current follows. The latter, delayed current (Id) is insensitive to NPPB, a nonspecific blocker of Clâ channels, and intracellular Mg2+, which inhibits VRAC and swelling-activated TRPM3 and TRPM7 channels. Replacement of extracellular Na+ with NMDG+ reduces inward tail currents, suggesting that Id is mediated by cations. Finally, Id shows voltage-dependent activation with slow activation kinetics and half-maximal activation at +76 mV. These pharmacological and biophysical characteristics of Id are distinct from those of known osmotic cell swelling-activated ion channels. In conclusion, our data identify and characterize a novel osmotically-activated, voltage-dependent ion channel in N2a cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 2, 4 March 2017, Pages 331-335
Journal: Biochemical and Biophysical Research Communications - Volume 484, Issue 2, 4 March 2017, Pages 331-335
نویسندگان
Akiyuki Taruno, Yoshinori Marunaka,