کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5505948 | 1400282 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Affinity chromatography of a binder of 1-methyladenine, the maturation-inducing hormone for starfish oocytes
ترجمه فارسی عنوان
کروماتوگرافی وابستگی یک اتصال دهنده 1-متیل آدنین، هورمون القا کننده بلوغ برای تخمک های دریایی ستاره دریایی
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کلمات کلیدی
HEPESEGTASDSMPFEC50CBBPAGEPMSFASWGVBDMIH1-methyladenine - 1-متیل آدنین2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid - 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acidcoomassie brilliant blue R-250 - coomassie درخشان آبی R-250polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدoocyte maturation - بلوغ تخمک یا بلوغ اووسیتmaturation-promoting factor - بلوغ فاکتور ترویجStarfish - ستاره دریاییsodium dodecyl sulfate - سدیم دودسیل سولفاتPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMaturation-inducing hormone - هورمون القاء بلوغAffinity chromatography - کروماتوگرافی میل ترکیبیReceptor - گیرنده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Starfish oocytes are arrested at the prophase stage of the first meiotic division in the ovary. They resume meiosis by the stimulus of 1-methyladenine (1-MeAde), the maturation-inducing hormone for starfish oocytes. Putative 1-MeAde receptors have been suggested to be present on the oocyte surface, but not yet been characterized biochemically. As reported recently (T. Toraya, T. Kida, A. Kuyama, S. Matsuda, S. Tanaka, Y. Komatsu, T. Tsurukai, Biochem. Biophys. Res. Commun. 485 (2017) 41-46), it became possible to detect unknown 1-MeAde binders of starfish oocytes by immunophotoaffinity labeling, i.e., photoaffinity labeling combined with immunochemical detection. We designed and synthesized water-soluble and insoluble polymer-bound 1-MeAde derivatives. A water-soluble polymer-bound 1-MeAde derivative, in which 1-MeAde is bound to dextran through an N6-substituent, triggered the germinal-vesicle breakdown toward follicle-free oocytes, dejellied oocytes, and denuded oocytes. This is consistent with the idea that putative 1-MeAde receptors are located on the cell surface of starfish oocytes. A water-insoluble polymer-bound 1-MeAde derivative, in which 1-MeAde is bound to Sepharose 4B through an N6-substituent, served as an effective affinity adsorbent for the partial purification of a 1-MeAde binder with Mr of 47.5Â K that might be a possible candidate of the maturation-inducing hormone receptors of starfish oocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 4, 13 May 2017, Pages 1055-1061
Journal: Biochemical and Biophysical Research Communications - Volume 486, Issue 4, 13 May 2017, Pages 1055-1061
نویسندگان
Tetsuo Toraya, Atsushi Kuyama, Seiichi Tanaka, Masatoyo Yamamoto, Tadamasa Ohmiya, Yuri Saito, Tomoko Tanabe,