کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5506674 | 1400301 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes
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کلمات کلیدی
DCsdendritic cell immunoreceptorMLNsDCIRSLNsIFN-γmAbcDCs - cdc هاMonoclonal antibody - آنتی بادی مونوکلونالinterferon-γ - اینترفرون-γDifferentiation - تفکیکconventional dendritic cells - سلول های دندریتی معمولیDendritic cells - سلول های دندریتیکLectin - لکتینbone marrow - مغز استخوانMonocytes - مونوسیتهاMarker - نشانگرmesenteric lymph nodes - گره های لنفاوی مزانتریک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes Dendritic cell inhibitory receptor 4 (DCIR4) is preferentially expressed on inflammatory and patrolling monocytes](/preview/png/5506674.png)
چکیده انگلیسی
Dendritic cell inhibitory receptor 4 (DCIR4, Clec4a1) is a lectin receptor and a member of mouse dendritic cell immunoreceptor family. Due to the lack of antibodies against DCIR4, expression of DCIR4 protein remains unknown. In this study, we established a specific monoclonal antibody against DCIR4 and investigated the expression of DCIR4 among immune cells. We found that DCIR4 was expressed on non-granulocytic subsets of CD11b+ cells in various immune organs including bone marrow, peripheral blood, spleen, skin-associated lymph nodes and mesenteric lymph nodes. DCIR4+ CD11b+ cells were dichotomized into DCIR4High and DCIR4Low cells distinguished by different levels of DCIR4 expression. By screening a panel of cell surface markers for expression, we found that in bone marrow, blood and spleen the DCIR4Low and the DCIR4High cells were Ly-6C+ CD43Low CD11câ inflammatoryâ monocytes and Ly-6Câ CD43HIgh CD11c+ patrolling monocytes, respectively. Using in vitro differentiation system, we also found that differentiation of Ly-6C+ monocytes into dendritic cells greatly diminished expression of DCIR4, while that into macrophages did not significantly affect DCIR4 expression. The establishment of the anti-DCIR4 antibody enables clearer definition of monocytes and provides a novel tool to investigate biology of monocytes and their progenies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 480, Issue 2, 11 November 2016, Pages 215-221
Journal: Biochemical and Biophysical Research Communications - Volume 480, Issue 2, 11 November 2016, Pages 215-221
نویسندگان
Yosuke Kameda, Motoki Hanayama, Atsushi Kishimoto, Masahiko Kume, Kazuo Yamamoto, Naoki Matsumoto,