کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5507195 | 1400317 | 2017 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectral dependence of irreversible light-induced fluorescence quenching: Chlorophyll forms with maximal emission at 700-702 and 705-710Â nm as spectroscopic markers of conformational changes in the core complex
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کلمات کلیدی
ChlNPQDCMUDBMIB3-(3,4-Dichlorophenyl)-1,1-dimethylurea - 3- (3،4-Dichlorophenyl) -1،1-dimethylureaFluorescence emission - انتشار فلورسانسLHC - برخورد دهنده بزرگ هادرون Non-photochemical quenching - خنک سازی غیر فتوشیمیاییChlorophyll - سبزینه یا کلروفیلPhotosystem II - فتوسیستم 2Light harvesting complex - مجتمع برداشت نورCore complex - مجتمع هسته ایReaction centre - مرکز واکنش
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Spectral dependence of irreversible light-induced fluorescence quenching: Chlorophyll forms with maximal emission at 700-702 and 705-710Â nm as spectroscopic markers of conformational changes in the core complex Spectral dependence of irreversible light-induced fluorescence quenching: Chlorophyll forms with maximal emission at 700-702 and 705-710Â nm as spectroscopic markers of conformational changes in the core complex](/preview/png/5507195.png)
چکیده انگلیسی
The spectral dependence of the irreversible non-photochemical fluorescence quenching associated with photoinhibition in vitro has been comparatively investigated in thylakoid membranes, PSII enriched particles and PSII core complexes isolated from spinach. The analysis of the fluorescence emission spectra of dark-adapted and quenched samples as a function of the detection temperature in the 280-80Â K interval, indicates that Chlorophyll spectral forms having maximal emission in the 700-702Â nm and 705-710Â nm ranges gain relative intensity in concomitance with the establishment of irreversible light-induced quenching, acting thereby as spectroscopic markers. The relative enhancement of the 700-702Â nm and 705-710Â nm forms emission could be due either to an increase of their stoichiometric abundance or to their intrinsically low fluorescence quantum yields. These two factors, that can also coexist, need to be promoted by light-induced alterations in chromophore-protein as well as chromophore-chromophore interactions. The bands centred at about 701 and 706Â nm are also observed in the PSII core complex, suggesting their, at least partial, localisation in proximity to the reaction centre, and the occurrence of light-induced conformational changes in the core subunits.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1858, Issue 7, July 2017, Pages 529-543
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1858, Issue 7, July 2017, Pages 529-543
نویسندگان
Sherzod Nematov, Anna Paola Casazza, William Remelli, Vakhobjon Khuvondikov, Stefano Santabarbara,