کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5508370 | 1537687 | 2017 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nanotubes connecting B lymphocytes: High impact of differentiation-dependent lipid composition on their growth and mechanics
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کلمات کلیدی
AFMGlucosyl ceramideCTX-BLacCerglycosphingolipidGlcCerGSLGM3CERTMbCDECMPI(4,5)P2 - PI (4،5) P2sphingolipids - اسفنگولیپیدsphingomyelin - اسفنگومیلینdocosahexaenoic acid - اسید داکوزاگزوائونیکLinoleic acid - اسید لینولئیکPolyunsaturated fatty acid - اسید چرب غیر اشباعPUFA - اسید چرب چند غیراشباعDHA - دوکوساهگزائنوئیک اسیدCer - سرceramide - سرامیدPlasma membrane - غشای پلاسماLipidomics - لیپیدومیکسExtracellular matrix - ماتریکس خارج سلولیmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینMembrane mechanics - مکانیک غشاءatomic force microscopy - میکروسکوپ نیروی اتمیNanotube - نانولولهcholesterol - کلسترولcholera toxin B subunit - کلسترول توکسین B واحد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Nanotubes (NTs) are thin, long membranous structures forming novel, yet poorly known communication pathways between various cell types. Key mechanisms controlling their growth still remained poorly understood. Since NT-forming capacity of immature and mature B cells was found largely different, we investigated how lipid composition and molecular order of the membrane affect NT-formation. Screening B cell lines with various differentiation stages revealed that NT-growth linearly correlates with membrane ganglioside levels, while it shows maximum as a function of cholesterol level. NT-growth of B lymphocytes is promoted by raftophilic phosphatidylcholine and sphingomyelin species, various glycosphingolipids, and docosahexaenoic acid-containing inner leaflet lipids, through supporting membrane curvature, as demonstrated by comparative lipidomic analysis of mature versus immature B cell membranes. Targeted modification of membrane cholesterol and sphingolipid levels altered NT-forming capacity confirming these findings, and also highlighted that the actual lipid raft number may control NT-growth via defining the number of membrane-F-actin coupling sites. Atomic force microscopic mechano-manipulation experiments further proved that mechanical properties (elasticity or bending stiffness) of B cell NTs also depend on the actual membrane lipid composition. Data presented here highlight importance of the lipid side in controlling intercellular, nanotubular, regulatory communications in the immune system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1862, Issue 9, September 2017, Pages 991-1000
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1862, Issue 9, September 2017, Pages 991-1000
نویسندگان
Eszter A. Tóth, Ádám Oszvald, Mária Péter, Gábor Balogh, Anikó Osteikoetxea-Molnár, Tamás Bozó, Edina Szabó-Meleg, Miklós Nyitrai, Imre Derényi, Miklós Kellermayer, Toshiyuki Yamaji, Kentaro Hanada, László VÃgh, János Matkó,