کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5509178 1538403 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperStructural and functional characterization of complex formation between two Kunitz-type serine protease inhibitors from Russell's Viper venom
ترجمه فارسی عنوان
ویژگی های ساختاری و عملکردی تشکیل پیچیده بین دو مهارکننده پروتئاز سریین از نوع کانینیتس از زهر ویبره راسل
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- Structural evidence of Rusvikunin complex formation in RVV has been shown.
- Rusvikunin complex consists of Rusvikunin and Rusvikunin II at 1:2 stoichiometry.
- Anticoagulant activity of the complex surpasses their individual potencies.

Snake venom Kunitz-type serine protease inhibitors (KSPIs) exhibit various biological functions including anticoagulant activity. This study elucidates the occurrence and subunit stoichiometry of a putative complex formed between two KSPIs (Rusvikunin and Rusvikunin-II) purified from the native Rusvikunin complex of Pakistan Russell's Viper (Daboia russelii russelii) venom (RVV). The protein components of the Rusvikunin complex were identified by LC-MS/MS analysis. The non-covalent interaction between two major components of the complex (Rusvikunin and Rusvikunin-II) at 1:2 stoichiometric ratio to form a stable complex was demonstrated by biophysical techniques such as spectrofluorometric, classical gel-filtration, equilibrium gel-filtration, circular dichroism (CD), dynamic light scattering (DLS), RP-HPLC and SDS-PAGE analyses. CD measurement showed that interaction between Rusvikunin and Rusvikunin-II did not change their overall secondary structure; however, the protein complex exhibited enhanced hydrodynamic diameter and anticoagulant activity as compared to the individual components of the complex. This study may lay the foundation for understanding the basis of protein complexes in snake venoms and their role in pathophysiology of snakebite.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volumes 128–129, September–October 2016, Pages 138-147
نویسندگان
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