کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5509395 | 1538513 | 2017 | 38 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High glucose down-regulates microRNA-181a-5p to increase pro-fibrotic gene expression by targeting early growth response factor 1 in HK-2 cells
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کلمات کلیدی
EGR1DKDRenal tubulointerstitial fibrosisHK-2 cellHK-2TIFOLETFECMHEK293qRT-PCRDMEMTGF-β1FBSDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoESRD یا end stage renal disease - بیماری کلیوی در مرحله نهایی End-stage renal disease - بیماری کلیوی در مرحله پایانیDiabetic kidney disease - بیماری کلیوی دیابتیTransforming growth factor β1 - تبدیل فاکتور رشد β1LETO - سالfetal bovine serum - سرم جنین گاوhuman embryonic kidney 293 cell line - سلول انسانی جنین 293 سلولی استearly growth response factor 1 - فاکتور رشد اولیه رشد 1Tubulointerstitial fibrosis - فیبروز توموگرافیFibronectin - فیبرونکتینExtracellular matrix - ماتریکس خارج سلولیUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهDiabetic nephropathy - نفروپاتی دیابتیquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استCol I - کل منCollagen I - کلاژن منhigh glucose - گلوکز بالا یا قند بالاnormal glucose - گلوکز طبیعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tubulointerstitial fibrosis (TIF) plays an important role in the progression of renal fibrosis in diabetic nephropathy (DN). Accumulating evidence supports a crucial effect of early growth response factor 1 (Egr1) on renal fibrosis in DN, but the underlying mechanisms are not entirely clear. Here, we explored the aggravating role of Egr1 and identified microRNA-181a-5p (miR-181a-5p) as an upstream regulator of Egr1 in TIF of DN. We demonstrated that overexpression of Egr1 enhanced, whereas small interfering RNA targeting Egr1 decreased the expressions of transforming growth factor β1 (TGF-β1) and fibrosis-related genes including fibronectin and collagen I in human proximal tubule cell line (HK-2) cells. We then found that miR-181a-5p expression was down-regulated, accompanied by the corresponding up-regulation of Egr1, TGF-β1, fibronectin and collagen I in renal tissues of type 2 diabetic Otsuka-Long-Evans-Tokushima-Fatty rats with DN, and that the expression of miR-181a-5p was negatively correlated with the level of Egr1 in HK-2 cells treated with high glucose. Furthermore, we identified that miR-181a-5p directly suppressed Egr1 to decrease the expressions of TGF-β1, fibronectin and collagen I in HK-2 cells through targeting the 3Ⲡuntranslated region of Egr1. The functional relevance of miR-181a-5p-induced Egr1 decrease was supported by inhibition and overexpression of miR-181a-5p in HK-2 cells. Thus, we concluded that aberrant Egr1 expression, which can be suppressed by miR-181a-5p directly, plays a crucial role in the progression of renal TIF in DN. This study indicates that targeting miR-181a-5p may be a novel therapeutic approach of DN.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 31, February 2017, Pages 96-104
Journal: Cellular Signalling - Volume 31, February 2017, Pages 96-104
نویسندگان
Ping Xu, Mei-ping Guan, Jian-gang Bi, Dan Wang, Zong-ji Zheng, Yao-ming Xue,