کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511254 | 1539849 | 2017 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A T cell-specific knockout reveals an important role for protease-activated receptor 2 in lymphocyte development
ترجمه فارسی عنوان
تکثیر تک سلولی مشخص کننده نقش مهمی در گیرنده 2 پروتئاز فعال در رشد لنفوسیت ها
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کلمات کلیدی
MNEDAPIFSC-AFSC-HQPCSSC-A4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولCell differentiation - تمایز سلولیPar - توسطThymus - تیموسDouble negative - دو برابر منفی استT cells - سلول های تیKnockout mice - موش نابود شدهcell surface molecules - مولکول های سطح سلولیprotease-activated receptors - گیرنده فعال فعال پروتئازprotease-activated receptor - گیرنده پروتئاز فعال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Activation of protease-activated receptor-2 (PAR2) expressed by T cells has been linked to the bone loss associated with periodontitis. We generated PAR2 conditional-null mice and crossed these with mice expressing Cre recombinase under control of the Lck proximal promoter, to produce T cell-specific PAR2-null mice in order to further study the cellular mechanism involved in periodontitis. Here we report that efficient deletion of PAR2 in thymocytes isolated from T cell-specific PAR2-null mice resulted in thymic and splenic hypoplasia and a reduction in the cells of the cortex and a loss of distinction between the cortex and the medulla of the thymus. FACS analysis confirmed significant reductions in CD4 and CD8 double negative (DN3 and DN4) sub-populations, as well as double positive and single positive T cells, in T cell-specific PAR2-null mice compared to Cre expressing PAR2 wild-type mice. The proportion of annexin V positive and propidium iodide negative cells was increased in CD4 and CD8 double negative, double positive and single positive T cells from T cell-specific PAR2-null mice. No change in the proportion of Ki67 positive cells was observed in sections of thymus from T cell-specific PAR2-null mice, suggesting that the depletion of T cell sub-populations in T cell-specific PAR2-null mice resulted from increased apoptosis rather than reduced proliferation. Together, these results demonstrate that PAR2 plays an important and previously unrecognised anti-apoptotic role in T cell development and suggest that the PAR2 conditional-null mouse will be an important resource for determining tissue and cell specific effects of PAR2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 92, November 2017, Pages 95-103
Journal: The International Journal of Biochemistry & Cell Biology - Volume 92, November 2017, Pages 95-103
نویسندگان
Nidhish Francis, Alison L. Every, Babatunde A. Ayodele, Robert N. Pike, Eleanor J. Mackie, Charles N. Pagel,