کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5511292 | 1539851 | 2017 | 57 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Methylglyoxal synthase regulates cell elongation via alterations of cellular methylglyoxal and spermidine content in Bacillus subtilis
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کلمات کلیدی
CFPactin-like proteinspeAFtsZSpeBODCOrnithine decarboxylaseMGSPAGEDHAPGSHGFPMethylglyoxal synthasePBSORFSPEBacillus subtilismreB - MreBUV–Vis - UV-Visarginine decarboxylase - آرژینین دکربوکسیلازAgmatinase - آگماتینازSolid-phase extraction - استخراج فاز جامدSpermidine - اسپرمیدینSpermidine synthase - اسپنتیدین سنتازUltraviolet-visible - اشعه ماوراء بنفش قابل مشاهده استpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدdihydroxyacetone phosphate - دی هیدروکسی استون فسفاتAge - سنCell elongation - طول عمر سلولیopen reading frame - قاب خواندن بازlysine decarboxylase - لیزین دکربوکسیلازMethylglyoxal - متیل گلی اکسالAdvanced glycation end-product - محصول نهایی پیشرفته glycationPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریgreen fluorescent protein - پروتئین فلورسنت سبزcyan fluorescent protein - پروتئین فلورسنت سیانوژنHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Methylglyoxal regulates cell division and differentiation through its interaction with polyamines. Loss of their biosynthesizing enzyme causes physiological impairment and cell elongation in eukaryotes. However, the reciprocal effects of methylglyoxal and polyamine production and its regulatory metabolic switches on morphological changes in prokaryotes have not been addressed. Here, Bacillus subtilis methylglyoxal synthase (mgsA) and polyamine biosynthesizing genes encoding arginine decarboxylase (SpeA), agmatinase (SpeB), and spermidine synthase (SpeE), were disrupted or overexpressed. Treatment of 0.2 mM methylglyoxal and 1 mM spermidine led to the elongation and shortening of B. subtilis wild-type cells to 12.38 ± 3.21 μm (P < 0.05) and 3.24 ± 0.73 μm (P < 0.01), respectively, compared to untreated cells (5.72 ± 0.68 μm). mgsA-deficient (mgsAâ) and -overexpressing (mgsAOE) mutants also demonstrated cell shortening and elongation, similar to speB- and speE-deficient (speBâ and speEâ) and -overexpressing (speBOE and speEOE) mutants. Importantly, both mgsA-depleted speBOE and speEOE mutants (speBOE/mgsAâ and speEOE/mgsAâ) were drastically shortened to 24.5% and 23.8% of parental speBOE and speEOE mutants, respectively. These phenotypes were associated with reciprocal alterations of mgsA and polyamine transcripts governed by the contents of methylglyoxal and spermidine, which are involved in enzymatic or genetic metabolite-control mechanisms. Additionally, biophysically detected methylglyoxal-spermidine Schiff bases did not affect morphogenesis. Taken together, the findings indicate that methylglyoxal triggers cell elongation. Furthermore, cells with methylglyoxal accumulation commonly exhibit an elongated rod-shaped morphology through upregulation of mgsA, polyamine genes, and the global regulator spx, as well as repression of the cell division and shape regulator, FtsZ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 91, Part A, October 2017, Pages 14-28
Journal: The International Journal of Biochemistry & Cell Biology - Volume 91, Part A, October 2017, Pages 14-28
نویسندگان
Sang-Min Shin, Sung-Hyun Song, Jin-Woo Lee, Min-Kyu Kwak, Sa-Ouk Kang,