کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5513242 | 1540984 | 2016 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The vitamin D-dependent transcriptome of human monocytes
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کلمات کلیدی
direct repeat spaced by 3 nucleotidesCyPChIP-Seq1,25(OH)2D3 or 1,25DCCLCathelicidinCTCFchemokine (C–C motif) ligandFAIRE-seqDR31α,25-dihydroxyvitamin D3 - 1α، 25-دی هیدروکسوییتامین D325(OH)D3 - 25 (OH) D325-hydroxyvitamin D3 - 25 هیدروکسی ویتامین D3cAMP - cAMPfold change - تغییر در برابرformaldehyde-assisted isolation of regulatory elements sequencing - جداسازی فرمالدئید از عناصر تنظیم کننده عناصرcluster of differentiation - خوشه تمایزCytochrome P450 - سیتوکروم پی۴۵۰CCCTC-binding factor - عامل اتصال دهنده CCCTC
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Monocytes are important cells of the innate immune system that can differentiate into macrophages and dendritic cells. The biologically active form of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), serves as a ligand of the nuclear receptor vitamin D receptor (VDR). A key physiological function of 1,25(OH)2D3 is the defense against pathogens, such as those causing tuberculosis, that involves the modulation of the monocyte transcriptome. THP-1 cells are an established model of human monocytes, for which the at present largest set of 1,25(OH)2D3-affected genome-wide data are available. Here we summarize the insight obtained from the recent transcriptome of 1,25(OH)2D3-stimulated THP-1 cells, that was determined by triplicate RNA sequencing (RNA-seq). Primary and secondary vitamin D target genes being up- and down-regulated were related to changes in the epigenome of THP-1 cells, such as 1,25(OH)2D3-dependent chromatin opening and modulation of the genome-wide association of the transcription factors VDR and CCCTC-binding factor (CTCF) with their respective genomic binding sites. The anti-microbial response is the top-ranking early physiological function represented by 1,25(OH)2D3-stimulated genomic regions and genes, but also other immunity-related pathways, such as IL10 signaling, are activated. Taken together, the epigenomic and transcriptomic responses of THP-1 cells to 1,25(OH)2D3 represent a master example of the impact of vitamin D on human physiology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 164, November 2016, Pages 180-187
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 164, November 2016, Pages 180-187
نویسندگان
Antonio Neme, Veijo Nurminen, Sabine Seuter, Carsten Carlberg,