کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5513447 1541212 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative telomeric chromatin isolation protocol for human cells
ترجمه فارسی عنوان
پروتئین جداسازی کروماتین تلومر برای سلول های انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


- A method, termed QTIP to purify telomeric chromatin is described.
- QTIP allows comprehensive identification of telomeric proteins by mass spectrometry.
- QTIP includes SILAC-labeling to compare different telomeric states.

The ends of eukaryotic chromosomes, known as telomeres, consist of repetitive DNA sequences, multiple proteins and noncoding RNAs. Telomeres are dynamic structures that play crucial roles as guardians of genome stability and tumor suppressors. Defects in telomere length or protein composition can accelerate aging and are seen in telomere syndromes, which affect various proliferative tissues such as the bone marrow or the lungs. One of the biggest challenges in the telomere field is to identify the molecular changes at telomeres that occur during normal development, in cancer and in telomere syndromes. To tackle this problem, our laboratory has established a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified and analyzed by mass spectrometry. QTIP involves stable isotope labeling by amino acids in cell culture (SILAC) to compare and identify quantitative differences in telomere protein composition of cells from various states.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 114, 1 February 2017, Pages 28-38
نویسندگان
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