A chemiluminescent platform for smartphone monitoring of H2O2 in human exhaled breath condensates

- The peroxyoxalate chemiluminescent reaction enables smartphone detection of airway H2O2.
- Optimization of reagents and camera settings provides a 264 nM detection limit.
- Measurements of H2O2 in exhaled breath condensates match the Amplex Red assay.
- Described methods offer low cost monitoring of ROS in human airways.

Noninvasive measurement of oxidative markers in clinical samples has the potential to rapidly provide information for disease management, but is limited by the need for expensive analytical instrumentation that precludes home monitoring or point-of-care applications. We have developed a simple to use diagnostic platform for airway hydrogen peroxide (H2O2) that combines optimized reaction-based chemiluminescent designs with an inexpensive home-built darkbox and readily available smartphone cameras. Specialized photography software applications and analysis of pixel intensity enables quantification of sample concentrations. Using this platform, sample H2O2 concentrations as low as 264 nM can be detected. The platform has been used to measure H2O2 in the exhaled breath condensates of human subjects, showing good agreement with the standard Amplex Red assay.

159