کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5515525 | 1541910 | 2017 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research articleMap-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa) Research articleMap-based cloning and characterization of the novel yellow-green leaf gene ys83 in rice (Oryza sativa)](/preview/png/5515525.png)
- Gene ys83 is a novel yellow-green leaf gene in rice.
- Protein YS83 is targeted to the chloroplast.
- ys83 mutant phenotype resulted from loss-of-function of gene YS83.
- Gene ys83 could be used as a trait marker gene in commercial hybrid rice production.
Leaf-color mutants have been extensively studied in rice, and many corresponding genes have been identified up to now. However, leaf-color mutation mechanisms are diverse and still need further research through identification of novel genes. In the present paper, we isolated a leaf-color mutant, ys83, in rice (Oryza sativa). The mutant displayed a yellow-green leaf phenotype at seedling stage, and then slowly turned into light-green leaf from late tillering stage. In its yellow leaves, photosynthetic pigment contents significantly decreased and the chloroplast development was retarded. The mutant phenotype was controlled by a recessive mutation in a nuclear gene on the short arm of rice chromosome 2. Map-based cloning and sequencing analysis suggested that the candidate gene was YS83 (LOC_Os02g05890) encoding a protein containing 165 amino acid residues. Gene YS83 was expressed in a wide range of tissues, and its encoded protein was targeted to the chloroplast. In the mutant, a T-to-A substitution occurred in coding sequence of gene YS83, which caused a premature translation of its encoded product. By introduction of the wild-type gene, the ys83 mutant recovered to normal green-leaf phenotype. Taken together, we successfully identified a novel yellow-green leaf gene YS83. In addition, number of productive panicles per plant and number of spikelets per panicle only reduced by 6.7% and 7.6%, respectively, meanwhile its seed setting rate and 1000-grain weight (seed size) were not significantly affected in the mutant, so leaf-color mutant gene ys83 could be used as a trait marker gene in commercial hybrid rice production.
Journal: Plant Physiology and Biochemistry - Volume 111, February 2017, Pages 1-9