An easy method for bacterial expression and purification of wild-type and mutant superoxide dismutase 1 (SOD1)

- Expression of untagged wild-type and mutant human superoxide dismutase 1 (SOD1) proteins in the periplasm of E. coli.
- Simple and cheap purification of human SOD1 proteins from the periplasm of E. coli.
- Characterization of purified SOD1 proteins by SDS-PAGE and enzyme activity.
- Characterization of SOD1 aggregation by Thioflavin T binding, SDS-PAGE, electron microscopy, and cellular toxicity.